Non-invasive visualisation and identification of fluorescent Leishmania tarentolae in infected sand flies
نویسندگان
چکیده
منابع مشابه
Green fluorescent protein-tagged Leishmania in phlebotomine sand flies.
In this work we have used for the first time green fluorescent protein (GFP) tagged cells of the human parasite Leishmania donovani to observe its development in the gut of phlebotomine sand flies. Low numbers of GFP-tagged L. donovani were more easily detected than nontagged Leishmania, suggesting that GFP-tagged Leishmania could be used to efficiently study the biology of Leishmania in their ...
متن کاملDistribution and identification of sand flies naturally infected with Leishmania from the Southeastern Peruvian Amazon
BACKGROUND Cutaneous leishmaniasis (CL) is an important health problem in the New World affecting civilian and military populations that are frequently exposed in endemic settings. The Peruvian region of Madre de Dios located near the border with Brazil is one of the most endemic CL regions in South America with more than 4,451 reported cases between 2010 and 2015 according to the Peruvian epid...
متن کاملVisualisation of Leishmania donovani Fluorescent Hybrids during Early Stage Development in the Sand Fly Vector
BACKGROUND The Leishmania protozoan parasites cause devastating human diseases. Leishmania have been considered to replicate clonally, without genetic exchange. However, an accumulation of evidence indicates that there are inter-specific and intra-specific hybrids among natural populations. The first and so far only experimental proof of genetic exchange was obtained in 2009 when double drug re...
متن کاملSeasonal Variation in the Prevalence of Sand Flies Infected with Leishmania donovani
BACKGROUND Visceral Leishmaniasis (VL) is a life threatening neglected infectious disease in the Indian subcontinent, transmitted by the bite of female sand flies. Estimation of the infectivity in the vector population, collected in different seasons, may be useful to better understanding the transmission dynamics of VL as well as to plan vector control measures. METHODOLOGY We collected sand...
متن کاملA lipophosphoglycan-independent development of Leishmania in permissive sand flies.
Leishmaniases are serious parasitic diseases the etiological organisms of which are transmitted by insect vectors, phlebotominae sand flies. Two sand fly species, Phlebotomus papatasi and P. sergenti, display remarkable specificity for Leishmania parasites they transmit in nature, but many others are broadly permissive to the development of different Leishmania species. Previous studies have su...
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ژورنال
عنوان ژورنال: Wellcome Open Research
سال: 2018
ISSN: 2398-502X
DOI: 10.12688/wellcomeopenres.14910.1